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Week
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Topics
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Study Metarials
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1
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Course description: Introduction and learning objectives
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R1- Chapter-1
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2
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Introduction of tools and equipment used in molecular biology laboratories
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R1- Chapter-1
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3
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Isolation and determination of DNA from blood
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R1- Chapter-2
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4
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Isolation and determination of DNA from tissue
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R1- Chapter-2
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5
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Preparation of Polymerase Chain Reaction mix, mix dstribution
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R1- Chapter-5
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6
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PCR amplification
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R1- Chapter-5
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7
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Agarose Gel preparation, PCR Product Application
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R1- Chapter-2
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8
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Acrylamide Gel Electrophoresis
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R1- Chapter-2
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9
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Electrophoresis Result Interpretation
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R1- Chapter-2
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10
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Quantitiy of nucleic acid
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R1- Chapter-2
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11
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RNA isolation
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R1- Chapter-3
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12
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cDNA synthesis
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R1- Chapter-3
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13
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Real time PCR mix preparation, distribution
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R2- Chapter-19
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14
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Interpretation of Real Time PCR results
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R2- Chapter-19
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Prerequisites
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None
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Language of Instruction
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Turkish
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Responsible
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Assoc. Prof. Dr. Kubilay TOYRAN
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Instructors
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1-)Doçent Dr. Kubilay Toyran
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Assistants
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-
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Resources
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R1: Temizkan G., Yılmazer S., Öztürk M., Arı, Ş., Ertan, H., Topal-Sarıkaya, A., Arda, N. (2008). Moleküler Biyolojide Kullanılan Teknikler. (3. Baskı). Temizkan G. & Arda, N. (ed.), Nobel Tıp Yayınevi, Ankara.
R2: Bayraç, A.T., Koç, A., Bekki, G., Yıldırım, H., Sadi, G. (2012). Moleküler Biyoloji. (3. Baskı). Karataş, M. (ed.), Nobel Tıp Yayınevi, Ankara.
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Supplementary Book
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SR1: Temizkan G., Topal-Sarıkaya, A., Günel, T., Gözükırmızı, T., Arda, N. (2018). Temel ve İleri Moleküler Biyoloji Yöntemleri Genomik ve Proteomik Analizler. (1. Baskı). Temizkan G. & Arda, N. (ed.), Nobel Tıp Yayınevi, Ankara.
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Goals
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It is aimed to learn and apply the working principles of molecular biological methods used in medical laboratories for genetic diagnosis.
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Content
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Introduction of general tools and equipment used in molecular biology laboratory, working rules in molecular biology laboratories, isolation and amplification of DNA and RNA from blood and tissues, visualization and interpretation of amplified products in agarose gel electrophoresis, types of polymerase chain reaction, determination of nucleic acid quantity.
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Program Learning Outcomes |
Level of Contribution |
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1
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Has knowledge about medical terms and terminology related to his area and makes definitions.
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4
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2
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Evaluates and applies the basic scientific knowledge in his area.
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-
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3
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Uses medical laboratory tools and equipment in accordance with the rules and techniques, checks and maintains them.
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5
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4
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Applies safety rules in medical laboratories, takes personal safety precautions and creates a safe laboratory environment.
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4
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5
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Prepares the solution related to the tests in the laboratory, makes the samples ready for analysis, makes practical application.
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4
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6
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Defines the factors of infection and applies appropriate disinfection and sterilization techniques.
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-
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7
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Demonstrate the necessary knowledge and skills for obtaining, storing and transferring biological material from the patient for medical laboratory tests.
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-
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8
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By using the basic knowledge and skills acquired in the field of Medical Laboratory Techniques Program he interprets and evaluates the data, identifies, analyzes the problems and takes part in the studies planned for the solution / takes responsibility.
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-
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9
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Apply environmental health and waste management precautions in medical laboratory studies.
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-
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10
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Takes responsibility in individual or team and solves the problems that he faced.
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-
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11
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Improves himself by following scientific and technological developments related to his area.
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4
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12
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Acts in accordance with social, scientific, cultural and ethical values during data collection, interpretation, application and announcement of the results in his area.
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-
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13
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Explains the structure and mechanism of living things.
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-
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14
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Defines the mechanism of disease formation, knows and applies the basic diagnostic tests.
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-
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15
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Has the basic theoretical and practical knowledge in his area.
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-
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