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Week
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Topics
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Study Metarials
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1
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Introduction to the Biochemistry laboratory
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R1: CHAPTER 1, R2: CHAPTER 1
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2
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General principles of biochemical investigation
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R1: CHAPTER 2, R2: CHAPTER 1
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3
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Optical methods 1
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R1: CHAPTER 3, R2: CHAPTER 1
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4
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Optical methods 2
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R1: CHAPTER 7, R3: CHAPTER 5
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5
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Protein determination methods
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R1: CHAPTER 11, R3: CHAPTER 4
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6
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DNA, RNA, and phosphate determination methods
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R1: CHAPTER 9-10, R2: CHAPTER 4
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7
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Fluorometry
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R1: CHAPTER 7, R3: CHAPTER 5
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8
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Infrared spectroscopy
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R1: CHAPTER 6, R3: CHAPTER 5
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9
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Polarimeter
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R1: CHAPTER 7, R2: CHAPTER 6
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10
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Chromatographic methods for protein purification
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R1: CHAPTER 5, R3: CHAPTER 3
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11
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Thin Layer Chromatography
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R1: CHAPTER 5, R3: CHAPTER 3
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12
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Types of electrophoresis
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R1: CHAPTER 6, R3: CHAPTER 4
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13
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Centrifugation
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R1: CHAPTER 4, R3: CHAPTER 7
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14
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Seminars, assignments and discussion
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Prerequisites
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-
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Language of Instruction
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Turkish
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Responsible
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Assoc. Prof. Dr. Veyis KARAKOÇ
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Instructors
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1-)Doçent Veyis Karakoç
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Assistants
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-
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Resources
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R1: Boyer, R. (2012). Biochemistry laboratory: Modern theory and techniques, 2. Edition, Pearson, New Jersey, USA.
R2: Sengar, R. S. (2014). Laboratory Manual of Biochemistry: Methods and Techniques. New India Publishing AGEN.
R3: Boyer, R. (2000). Modern experimental biochemistry. Pearson Education India.
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Supplementary Book
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-
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Goals
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The aims of this course are to introduce students to the theoretical and practical aspects of advanced laboratory techniques in biochemistry.
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Content
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Introduction to the Biochemistry laboratory, General principles of biochemical investigation, Optical methods, Protein determination methods, DNA, RNA, and phosphate determination methods, Fluorometry, Infrared spectroscopy, Polarimeter, Chromatographic methods for protein purification, Thin Layer Chromatography, Types of electrophoresis, Centrifugation
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Program Learning Outcomes |
Level of Contribution |
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1
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Has the necessary theoretical and applied basic knowledge within the scope of natural science. Has the knowledge to evaluate the nature, source, limits, accuracy, reliability, and validity of the information.
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3
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2
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Participates in interdisciplinary studies by using the basic knowledge of the field and analytical thinking ability.
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-
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3
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Evaluates concepts, ideas, and data in the field of chemistry with scientific methods, identify and analyze complex problems and issues, make discussions, and develop suggestions based on evidence and research.
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4
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4
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Has the ability to design and implement experiments, use modern technical devices, collect data and analyze results in order to research and solve problems related to the field.
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-
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5
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Produces solutions by taking responsibility for solving unpredictable complex situations in applications in the field of chemistry.
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3
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6
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Informs periodically the people and institutions it is responsible for while conducting studies related to its field, and expresses its findings and suggestions for solutions to emerging problems in written, oral, and, if necessary, visual presentation.
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-
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7
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Determines the theoretical and practical deficiencies related to the field and directs the future learning processes.
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3
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8
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Has the knowledge of a foreign language at a level to be able to access the scientific information needs from foreign sources related to the field, update knowledge, and communicate with colleagues around the world.
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-
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9
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Uses computer software, information, and communication technologies at the level required by the field and accesses scientific resources in this way.
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-
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10
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Develops strategy, policy, and implementation plans on issues related to the field and manages the data obtained within the framework of quality processes.
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-
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11
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Supervises and checks the social, scientific, cultural, and ethical values at the stages of collection, interpretation, application, and announcement of the data related to the field.
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-
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