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Week
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Topics
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Study Metarials
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1
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Definition of plant biotechnology, history, and general presentation of the application areas. Introduction of the laboratory.
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R1 Sec 1 (1-35)
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2
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Fundamentals of botanical tissue culture. Safety of the laboratory.
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R2 Sec 1 (5-40)
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3
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Basic laboratory techniques. Basic tools, equipment and materials presentation. Basic laboratory techniques
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R2 Sec 3 (65-86)
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4
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Media and sterilization in tissue cultures. Sterilization.
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R2 Sec 2 (42-65)
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5
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Shoot tip and micrografting techniques. Meristem cultures. The preparation of inventories.
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R1 Sec 8 (262-281)
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6
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Organogenesis. The preparation of media.
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R1 Sec 2 (36-70)
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7
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Somatic embryogenesis. Plant explants place in media.
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R1 Sec 3 (71-88)
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8
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Synthetic seed production. Observation of plant growing.
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R2 Sec 5 (98-125)
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9
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Germplasm conservation. Observation of plant growing.
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R1 Sec 9 ( 282-323)
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10
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Somaclonal variations. Subcultures of plants.
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R1 Sec 11 (345-367)
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11
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Embryo culture. Observation of plant growing.
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R1 Sec 10 (324-344)
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12
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Protoplast culture and fusion. Rooting of plants.
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R1 Sec 4 (89-136)
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13
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Anther culture. Observation of plant growing.
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R1 Sec 5 (137-189)
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14
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Biosafety and ethics. Observation of plant growing.
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R2 Sec 10 (335-352)
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Prerequisites
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-
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Language of Instruction
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Turkish
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Responsible
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Assoc.Prof.Dr.Mehmet SEZGİN
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Instructors
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1-)Doçent Dr. Mehmet Sezgin
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Assistants
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-
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Resources
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R1) Babaoglu, M., Gürel, E. ve Özcan, S. (Eds.) 2002. Bitki Biyoteknolojisi. Vol. I-II. Doku Kültürü ve Uygulamaları. S.Ü. Basımevi, Konya.
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Supplementary Book
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R2) Dixon, R.A. 1991. Plant Cell and Tissue Culture: A Practical Approach. IRL Pres,Oxford.
George, F,E., Hall, M.A. and Jan de Klerk, G. (eds) 2008. Plant Propagation by Tissue Culture. 3rd Ed. Springer. Dordrecht. The Netherlands.
R3) Trigiano, R.N. and Gray, D.J. (Eds.) 2008. Plant Development and Biotechnology. CRC Press. London.
Geneve, R.L., Preece, J.E. and Merkle S.A. (eds) 2006. Biotechnology of ornamental Plants. Biotechnology in Agriculture Series No:16 CABI. UK.
R4) Mohan Jain, S., Brar, D.S. and Ahloowalia, B.S. (Eds.) 2002. Molecular Techniques in Crop Improvement. Kluwer Academic Publishers, London.
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Goals
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Intended uses of in vitro techniques in plants, fields and potentials, different woody and herbaceous plant species on the success of in vitro techniques genotype, explant, medium, influential factors, such as physical and cultural conditions and studies on herbaceous and woody plants and future goals subjects will be discussed.
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Content
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Definition of plant biotechnology, history, and general presentation of the application areas. Fundamentals of botanical tissue culture. Basic laboratory techniques. Basic tools, equipment and materials presentation.
Media and sterilization in tissue cultures. Shoot tip and micrografting techniques. Meristem cultures. Organogenesis. Somatic embryogenesis. Synthetic seed production. Germplasm conservation. Somaclonal variations. Embryo culture. Protoplast culture and fusion. Anther culture. Biosafety and ethics.
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Program Learning Outcomes |
Level of Contribution |
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1
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Improves the recent and advanced knowledge in the field to the level of expertise using original thought and/or research, deepens it, and reaches to the original definitions to bring novelty to the field.
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4
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2
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Evaluates the expertise-level theoretical and applied knowledge, obtained in the biology field, by a systematic approach and uses it.
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3
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3
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Interprets the knowledge in the biology field combining the knowledge from different disciplines, and generates novel knowledge.
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4
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4
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Analyzes a problem in the biology field independently, generates a solution method, and solves the problem evaluating the data.
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4
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5
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Develops novel approaches intended for solutions against the possible problems of applications in the biology field, and generates solution taking the responsibility.
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4
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6
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Gains ability for evaluating the knowledge in the biology field with a critical point of view, and for directing the learning.
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-
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7
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Follows the recent developments in the biology field and in own study, also transfers own studies systematically to the groups in the field and out-groups by written, verbal and visual means.
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-
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8
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Examines the social relations and the standards directing these relations by a critical aspect, and when needed, makes contributions to improve these standards.
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-
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9
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Follows the knowledge in the biology field using a foreign language, communicates verbally and in writing.
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4
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10
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Uses software, information and communication technologies required for the biology field, and reaches to the scientific resources in this way.
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-
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11
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Develops strategy, policy and application plans for the topics related with the biology field, and manages the data obtained evaluating them within the framework of quality processes.
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-
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12
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Supervises and checks the social, scientific, cultural and ethical values at the stages of collection, interpretation, application and announcement of the data related with the biology field.
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3
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13
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Identifies the interdiciplinary interaction related with the field, and uses the knowledge and skills obtained to generate solutions against possible problems encountered in the interdiciplinary team works.
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-
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14
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Uses the modern devices and techniques for the biology applications, and transfers the knowledge related with this topic.
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-
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15
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Publishes a scientific article related with the biology field in a national and/or international peer-reviewed journal.
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-
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