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  • Course Information
    • Course Title Code Semester Laboratory+Practice (Hour) Pool Type ECTS
    Bioanalytical Methods KİM605 FALL-SPRING 3+0 University E 6
    Learning Outcomes
    1-Defines bioanalytical chemistry and biomolecules.
    2-Selects the appropriate analysis method by using the properties of biomolecules.
    3-Explains the differences between various analytical methods used in the determination of biomolecules.
  • ECTS / WORKLOAD
    • ActivityPercentage

    (100)

    		NumberTime (Hours)Total Workload (hours)
    Course Duration (Weeks x Course Hours)14342
    Classroom study (Pre-study, practice)14570
    Assignments1011010
    Short-Term Exams (exam + preparation) 0000
    Midterm exams (exam + preparation)3013030
    Project0000
    Laboratory 0000
    Final exam (exam + preparation) 6014040
    0000
    Total Workload (hours)   192
    Total Workload (hours) / 30 (s)     6,4 ---- (6)
    ECTS Credit   6
  • Course Content
    • Week Topics Study Metarials
    1 Introduction to bioanalytical methods R1, 855-856 pp R2, 1-22 pp
    2 Physical, chemical and biological properties of biomolecules R1, 855-856 pp
    3 Biological sampling and sample preparation methods R2, 1-22 pp R3, 123-146 pp
    4 Selection of the appropriate analysis method using the properties of biomolecules R2, 1-22 pp
    5 Spectroscopic analysis of biomolecules R2, 131-590 pp
    6 Chromatographic analysis of biomolecules R2, 761-890 pp
    7 Electrochemical analysis of biomolecules R2, 627-750 pp
    8 Electrophoretic analysis of biomolecules R2, 867-888 pp
    9 Enzyme and substrate measurements R4, 59-70 pp
    10 Quantitative immunoassay techniques with antibodies and labeling method R5, 97-104 pp R6, 487-502 pp
    11 DNA, RNA-based techniques, PCR technique and applications R7, 99-119 pp R8, 983-992 pp
    12 Analysis of biomolecules with biosensors R9, 3-15 pp
    13 Bioanalytical validation R2, 967-990 pp
    14 Recent Developments in Bioanalytical Methods R10, 212-225 pp R11, 244-263 pp
    Prerequisites -
    Language of Instruction Turkish
    Responsible Assistant Prof. Dr. Melike BİLGİ KAMAÇ
    Instructors -
    Assistants -
    Resources R1. Larive, C. K. (2005). Instruction in bioanalytical chemistry. Analytical and bioanalytical chemistry, 382(4), 855-856. R2. Skoog, D. A., Holler, F. J., & Crouch, S. R. (2013). Enstrümental Analiz İlkeleri (E. Kılıç ve H. Yılmaz, Çev.). Ankara, Bilim yayıncılık. R3. Niu, Z., Zhang, W., Yu, C., Zhang, J., & Wen, Y. (2018). Recent advances in biological sample preparation methods coupled with chromatography, spectrometry and electrochemistry analysis techniques. TrAC Trends in Analytical Chemistry, 102, 123-146. R4. Morgan, M. T., & Wolberger, C. (2018). Competition assay for measuring deubiquitinating enzyme substrate affinity. In The Ubiquitin Proteasome System (pp. 59-70). Humana Press, New York, NY. R5. Aras, Z. (2011). Mikrobiyolojide kullanılan hızlı tanı yöntemleri. Türk Hijyen ve Deneysel Biyoloji Dergisi, 68(2), 97-104. R6. Hsieh, Y. P., & Rao, Q. (2017). Immunoassays. In Food Analysis (pp. 487-502). Springer, Cham. R7. Malapelle, U., Pisapia, P., Cieri, M., Pepe, F., & Troncone, G. (2019). RNA-Based Assays. In Molecular Diagnostics in Cytopathology (pp. 99-119). Springer, Cham. R8. Shahi, S., Vahed, S. Z., Fathi, N., & Sharifi, S. (2018). Polymerase chain reaction (PCR)-based methods: Promising molecular tools in dentistry. International journal of biological macromolecules, 117, 983-992. R9. Şahin M., "Etanol-metanol karısımının tayini için yüzey baskılı elektrotların modifiye edilmesiyle amperometrik alkol biyosensörünün gelistirilmesi, 2013, Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, (Doktora Tezi). R10. Pila?ová, V., Plachká, K., Khalikova, M. A., Svec, F., & Nováková, L. (2019). Recent developments in supercritical fluid chromatography mass spectrometry: Is it a viable option for analysis of complex samples. TrAC Trends in Analytical Chemistry, 112, 212-225. R11. Rood, J. J., Schellens, J. H., Beijnen, J. H., & Sparidans, R. W. (2016). Recent developments in the chromatographic bioanalysis of approved kinase inhibitor drugs in oncology. Journal of pharmaceutical and biomedical analysis, 130, 244-263.
    Supplementary Book -
    Goals It aims to recognize the bioanalytical methods used in the analysis of biomolecules and to learn the basic principles.
    Content Physical, chemical and biological properties of biomolecules; Biological sampling and sample preparation methods; Selection of the appropriate analysis method using the properties of biomolecules; Spectroscopic analysis of biomolecules; Chromatographic analysis of biomolecules; Electrochemical analysis of biomolecules; Enzyme and substrate measurements; Quantitative immunoassay techniques with antibodies and labeling method; DNA, RNA-based techniques, PCR technique and applications; Bioanalytical validation
  • Program Learning Outcomes
    • Program Learning Outcomes Level of Contribution
    1 Based on the competencies of the doctorate, to develop and deepen the current and advanced knowledge in the field with original thinking and research at the level of expertise and to reach original definitions that will bring innovation to science. 5
    2 Understanding the interaction between the disciplines related to the field of chemistry; to achieve original results by using expertise in analyzing, synthesizing and evaluating new and complex ideas. -
    3 To reach new scientific knowledge in the field of chemistry and to gain high level skills in research methods related to the field. 4
    4 To be able to develop a new scientific method in the field of chemistry or to apply a known method to a different problem. -
    5 To be able to research, comprehend, design, adapt and apply an original subject. -
    6 To be able to question, synthesize and evaluate new and complex ideas. 4
    7 Publishing original studies in peer-reviewed journals. -
    8 To be able to develop original ideas and methods related to the field and interdisciplinary by using high level mental processes such as creative and questioning thinking, problem solving and decision making -
    9 To be able to present their original views effectively in a professional community. -
    10 To be able to communicate and discuss in at least one foreign language at an advanced level, written, oral and visual. -
    11 To contribute to the process of becoming an information society by introducing technological advances in academic and professional contexts. -
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